Improvement of lipid production in oleaginous yeast Rhodosporidium toruloides by ultraviolet mutagenesis
نویسندگان
چکیده
منابع مشابه
Functional genomics of lipid metabolism in the oleaginous yeast Rhodosporidium toruloides.
The basidiomycete yeast Rhodosporidium toruloides (a.k.a. Rhodotorula toruloides) accumulates high concentrations of lipids and carotenoids from diverse carbon sources. It has great potential as a model for the cellular biology of lipid droplets and for sustainable chemical production. We developed a method for high-throughput genetics (RB-TDNAseq), using sequence-barcoded Agrobacterium tumefac...
متن کاملDynamics of the lipid droplet proteome of the Oleaginous yeast rhodosporidium toruloides.
Lipid droplets (LDs) are ubiquitous organelles that serve as a neutral lipid reservoir and a hub for lipid metabolism. Manipulating LD formation, evolution, and mobilization in oleaginous species may lead to the production of fatty acid-derived biofuels and chemicals. However, key factors regulating LD dynamics remain poorly characterized. Here we purified the LDs and identified LD-associated p...
متن کاملGenome sequence of the oleaginous red yeast Rhodosporidium toruloides MTCC 457.
We report the de novo assembled 20.05-Mb draft genome of the red yeast Rhodosporidium toruloides MTCC 457, predicted to encode 5,993 proteins, 4 rRNAs, and 125 tRNAs. Proteins known to be unique to oleaginous fungi are present among the predicted proteins. The genome sequence will be valuable for molecular genetic analysis and manipulation of lipid accumulation in this yeast and for developing ...
متن کاملHigh-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture
Microbial lipid production by the oleaginous yeast Rhorosporidium toruloides Y4 was studied using glucose as carbon source, in order to realize igh-density cell culture. Batch cultures demonstrated that there was little inhibitory effect with a substrate concentration of up to 150 g l−1. Flask ed-batch cultures were run for 25 days and reached a dry biomass and cellular lipid content of 151.5 g...
متن کاملDraft Genome Sequence of Rhodosporidium toruloides CECT1137, an Oleaginous Yeast of Biotechnological Interest
We report the sequencing of the basidiomycetous yeast Rhodosporidium toruloides CECT1137. The current assembly comprises 62 scaffolds, for a total size of ca. 20.45 Mbp and a G+C content of ca. 61.9%. The genome annotation predicts 8,206 putative protein-coding genes.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Engineering in Life Sciences
سال: 2019
ISSN: 1618-0240,1618-2863
DOI: 10.1002/elsc.201800203